A Culture Method for Lymnæa truncatul

Bag culture: a method for root-root co-culture.

A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts...

A simplified culture method for Blastocystis hominis

edigraphic.com Blastocystis hominis is a strict anaerobic protozoan that occurs in the intestine of humans and other mammals (Zierdt, 1991). Recently, phylogenetic analyses of ribosomal RNAs place B. hominis within the straminopiles (Silberman et al., 1996). Its pathogenic role in human beings is still disputed by some investigators (Markell and Udkow, 1986; Miller, 1988). The mechanism by whic...

Semiautomated method for quantitative urine culture.

Kemp RB. Effect of the removal of cell surface sialic acid on aggregation in vitro. Nature 1968;218: 1255. 8Steinhardt RA, Lundin L, Mazia D. Bioelectric responses of the echinoderm egg to fertilisation. Proc Natl Acad Sci 1971;68:2426-30. 9 Macieira-Coelho A, Avrameas S. Modulation of cell behaviour in vitro by the substratum in fibroblastic and leukaemic mouse cell lines. Proc Natl Acad Sci 1...

Modified method for fungal slide culture.

A modified slide culture method which combines advantages found in several slide culture methods is described. A block of inoculated nutrient agar sandwiched between two sterile cover glasses is placed in a plastic petri dish containing water agar. After adequate growth has occurred, the slide culture is disassembled and mounted in a conventional manner.

Limitations of semiquantitative method for catheter culture.